human bid peptide Search Results


human bid peptide  (GenScript corporation)
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GenScript corporation human bid peptide
( A ) Protein binding specificities of extant BCL-2 family members. Human MCL-1 (hsMCL-1, purple) strongly binds <t>BID</t> (blue) and NOXA (red), while human BCL-2 (hsBCL-2, green) strongly binds BID but not NOXA. ( B ) Crystal structures of MCL-1 (purple) bound to NOXA (red, PDB 2nla), and BCL-xL (green, a closely related paralog of BCL-2) bound to BID (blue, PDB 4qve). ( C ) Reduced maximum likelihood phylogeny of BCL-2 family proteins. Purple bar, MCL-1 class; green bar, BCL-2 class. The phylogeny was rooted using as outgroups the paralogs BOX, BAK, and BAX (black bar). Heatmaps indicate BID (blue) and NOXA (red) binding measured using the luciferase assay. Each shaded box shows the normalized mean of three biological replicates. Red dotted lines, interval during which NOXA binding was lost, yielding BID specificity in the BCL-2 proteins of vertebrates (green box). Purple box, vertebrate MCL-1. Silhouettes, representative species in each terminal group. AncMB1-M and -B are alternative reconstructions using different approaches to alignment ambiguity (see Materials and methods). For complete phylogeny, see .
Human Bid Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Protein binding specificities of extant BCL-2 family members. Human MCL-1 (hsMCL-1, purple) strongly binds BID (blue) and NOXA (red), while human BCL-2 (hsBCL-2, green) strongly binds BID but not NOXA. ( B ) Crystal structures of MCL-1 (purple) bound to NOXA (red, PDB 2nla), and BCL-xL (green, a closely related paralog of BCL-2) bound to BID (blue, PDB 4qve). ( C ) Reduced maximum likelihood phylogeny of BCL-2 family proteins. Purple bar, MCL-1 class; green bar, BCL-2 class. The phylogeny was rooted using as outgroups the paralogs BOX, BAK, and BAX (black bar). Heatmaps indicate BID (blue) and NOXA (red) binding measured using the luciferase assay. Each shaded box shows the normalized mean of three biological replicates. Red dotted lines, interval during which NOXA binding was lost, yielding BID specificity in the BCL-2 proteins of vertebrates (green box). Purple box, vertebrate MCL-1. Silhouettes, representative species in each terminal group. AncMB1-M and -B are alternative reconstructions using different approaches to alignment ambiguity (see Materials and methods). For complete phylogeny, see .

Journal: eLife

Article Title: Contingency and chance erase necessity in the experimental evolution of ancestral proteins

doi: 10.7554/eLife.67336

Figure Lengend Snippet: ( A ) Protein binding specificities of extant BCL-2 family members. Human MCL-1 (hsMCL-1, purple) strongly binds BID (blue) and NOXA (red), while human BCL-2 (hsBCL-2, green) strongly binds BID but not NOXA. ( B ) Crystal structures of MCL-1 (purple) bound to NOXA (red, PDB 2nla), and BCL-xL (green, a closely related paralog of BCL-2) bound to BID (blue, PDB 4qve). ( C ) Reduced maximum likelihood phylogeny of BCL-2 family proteins. Purple bar, MCL-1 class; green bar, BCL-2 class. The phylogeny was rooted using as outgroups the paralogs BOX, BAK, and BAX (black bar). Heatmaps indicate BID (blue) and NOXA (red) binding measured using the luciferase assay. Each shaded box shows the normalized mean of three biological replicates. Red dotted lines, interval during which NOXA binding was lost, yielding BID specificity in the BCL-2 proteins of vertebrates (green box). Purple box, vertebrate MCL-1. Silhouettes, representative species in each terminal group. AncMB1-M and -B are alternative reconstructions using different approaches to alignment ambiguity (see Materials and methods). For complete phylogeny, see .

Article Snippet: Peptide, recombinant protein , BID , GenScript , This Study , Human BID peptide used for fluorescence polarization (see Materials and methods).

Techniques: Protein Binding, Binding Assay, Luciferase

( A ) BID fluorescence polarization for hsMCL-1 variants evolved to lose NOXA binding. Bars are the mean of three replicates; error bars, SD. mFP, normalized measured fluorescent polarization. Kd estimates are shown below in the table. ( B ) Same as ( A ), but for NOXA binding. ( C ) BID fluorescence polarization for hsBCL-2 variants evolved to gain NOXA binding. ( D ) Same as ( C ), but for NOXA binding.

Journal: eLife

Article Title: Contingency and chance erase necessity in the experimental evolution of ancestral proteins

doi: 10.7554/eLife.67336

Figure Lengend Snippet: ( A ) BID fluorescence polarization for hsMCL-1 variants evolved to lose NOXA binding. Bars are the mean of three replicates; error bars, SD. mFP, normalized measured fluorescent polarization. Kd estimates are shown below in the table. ( B ) Same as ( A ), but for NOXA binding. ( C ) BID fluorescence polarization for hsBCL-2 variants evolved to gain NOXA binding. ( D ) Same as ( C ), but for NOXA binding.

Article Snippet: Peptide, recombinant protein , BID , GenScript , This Study , Human BID peptide used for fluorescence polarization (see Materials and methods).

Techniques: Fluorescence, Binding Assay

Journal: eLife

Article Title: Contingency and chance erase necessity in the experimental evolution of ancestral proteins

doi: 10.7554/eLife.67336

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , BID , GenScript , This Study , Human BID peptide used for fluorescence polarization (see Materials and methods).

Techniques: Recombinant, Fluorescence, Software